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Image Search Results
Journal: Journal of Biochemistry
Article Title: A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis
doi: 10.1093/jb/mvx039
Figure Lengend Snippet: Schematic representation of the tangential flow filtration (TFF) system used in the present study. (A) The system was manually controlled by using the pump output and adjustable valves 2 and 3 (V2 and V3) with modulating transmembrane pressure (TMP). TMP is the difference between the pressure upstream (applied pressure) and downstream (permeate) of the membrane, and is calculated using the equation TMP = [P feed (P1) + P retentate (P2)]/2 − P permeate (P3). The three pressure indicators are labeled as P1, P2 and P3. (B) Characterization of the membrane. The permeate flow (flux) of E. coli cell culture (OD 600 , ∼3.0) was measured by setting delta-P (P1 − P2) at 0.1 MPa and altering TMP in the range of 0–0.13 MPa.
Article Snippet: Alternatively, cells were harvested by using a
Techniques: Filtration, Membrane, Labeling, Cell Culture
Journal: Journal of Biochemistry
Article Title: A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis
doi: 10.1093/jb/mvx039
Figure Lengend Snippet: Diafiltration of S30 extract by means of tangential flow filtration. Representative results from a typical experiment using 300 ml of S30 extract preparation are shown. The MWCO value of the ultrafiltration membrane was 10 kDa. Optical density of the permeate was monitored by measuring absorbance at 260 nm. The dotted line shows the cumulative volume of permeate.
Article Snippet: Alternatively, cells were harvested by using a
Techniques: Diafiltration Assay, Filtration, Membrane